Composite

Part:BBa_K2557016:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-miniCMV promoter-TP901


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
    Illegal BamHI site found at 325
    Illegal BamHI site found at 1930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 391
    Illegal PstI site found at 964
    Illegal PstI site found at 1675
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 574
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of TP901 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References